Analysis Note
Clotting activity is determined by comparison to a standard curve produced using normal pooled human plasma
General description
Native coagulation factor V from human plasma. Thrombin cleaves Factor V to yield activated Factor Va, which exhibits 50-fold greater activity than the precursor molecule. Factor Va, Ca2+, and membrane phospholipids are cofactors which form a "prothrombinase" complex with Factor Xa, enabling its proteolytic activation of prothrombin to thrombin.
Native coagulation factor V from human plasma. Thrombin cleaves Factor V to yield activated Factor Va, which exhibits 50-fold greater activity than the precursor molecule. Factor Va, Ca2+, and membrane phospholipids are cofactors that form a "prothrombinase" complex with Factor X, enabling its proteolytic activation of prothrombin to thrombin.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Walker, F.J. 1992. J. Biol. Chem.267, 19896.Monkovic, D.D., and Tracy, P.B. 1990. J. Biol. Chem.265, 17132.Krishnaswamy, S., et al. 1989. J. Biol. Chem.264, 3160.Lee, C.D., and Mann, K.G. 1989. Blood73, 185.Krieg, U.C., et al. 1987. Biochemistry26, 103.
Packaging
100 µg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
Physical form
In 50% glycerol/H2O (v/v).
Preparation Note
Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C). Do not store below -20°C.
Warning
Toxicity: Standard Handling (A)
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